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Center for NMR Spectroscopy Washington State University

Instruments

Diagram of the WSU NMR Center

Varian Vx 300 MHz Spectrometer

All users are initially trained on this instrument. It is primarily used for 1H, 13C, 19F, and 31P NMR spectroscopy of solution-based room temperature samples. It is capable of 1D and 2D NMR experiments.

Technical Information

  • Bruker 7.05T, 54 mm bore magnet
  • Broadbanded 2 channel console
  • Nalorac 4-nucleus Plus 5mm probe, 1H-19F-13C-31P or 1H-X where X is tunable from 30 to 150 MHz
  • Modified Bruker Indirect detection probe, 1H-X where X is tunable over the range of 14 to 125 MHz
  • Variable temperature range of -150 °C to +150 °C

 

 

Varian 400 MHz Spectrometer

This instrument is the main workhorse of the NMR lab. Most users are trained on this instrument. It is primarily used for solution-based 1H, 13C, and multinuclear NMR spectroscopy. It is capable of 1D and 2D NMR experiments. The presence of an autosampler allows for automated data collection for data collection during off-peak hours.

Technical Information

  • Varian 400MR Console and 5mm OneProbe 2-channel autotuning probe
  • 15N to 31P tuning range (60 to 250 MHz) (Click here to see available nuclei)
  • Z-axis gradients
  • 96 position autosampler, full experiment automation
  • Variable temperature: -80 °C to +90 °C

 

 

Varian VNMRS Level 500 MHz Spectrometer

This instrument is one of the specialty instruments. It is primarily used for solid state, biological samples, HRMAS, 1H, 13C, and multinuclear NMR spectroscopy. It is capable of 1D and 2D NMR experiments. The age of the instrument requires for manual tuning of the spectrometer. The low 11B background in the broadband probe, makes this instrument the instrument of choice for compounds containing boron. This instrument is the easiest to reserve for long-term NMR experiments. This instrument is capable of analyzing biological in addition to agricultural samples (e.g. seeds, flowers, pollen, etc.) and is the instrument of choice for solid state and HRMAS analysis.

Technical Information

  • Varian VNMRS Console and various probes
  • Nalorac H{CN}Triple Resonance probe with Z-axis PFG–Mainly for biological NMR, protein structural analysis
  • Nalorac Dual Broad Band probe (tunable from 22 to 225 MHz with quartz inserts for low 11B background) with Z-axis PFG–manually tune X-channel for direct observation of heteronuclei with proton decoupling  (Click here to see available nuclei)
  • Nalorac HF dual tuned probe with Z-axis gradients (includes filters for {1H}19F and {19F}1H experiments)–primarily for fluorine NMR, but also has strongest gradient coils of any of our probes for DOSY experiments
  • Nalorac H{CPN} Quad Resonance probe with XYZ-axis PFG–specialty probe for phosphorus containing biological molecules
  • Varian High Resolution Magic Angle Spinning probe (Nanoprobe), two channel H-observe, X-decouple
  • Z-axis gradients
  • Oxford 11.75T, 51 mm magnet
  • Oxford 28 gradient shim set
  • 4 channel console, 1 full band channel and 3 broadband channels with waveform generators on each channel.
  • Triaxial Pulsed Field Gradient amplifier
  • Deuterium gradient shimming and decoupling switches
  • Variable temperature: -80 °C to +90 °C

Bruker Avance Neo 500 MHz Spectrometer

This instrument is the new instrument in the WSU Center for NMR Spectroscopy (arrived in November 2020 and installed in late January 2021). Users are trained on this instrument only upon request. It is primarily used for diffusion and solid state experiments, but it is also capable of solution-based 1H, 13C, and multinuclear NMR spectroscopy (1D and 2D NMR experiments). The presence of a 24 sample SampleCase autosampler allows for automated data collection for data collection during off-peak hours. We currently only have the iProbe available (we are waiting for the other probes to arrive and be validated).

  • Bruker iProbe two-channel: 1H and broadband (19F/31P–199Hg/17O– 109Ag),
  • Bruker TXI probe – triple resonance probe (1H, 15N, 13C)
  • Both probes have extended range beyond our current probes: up to 130 °C cooler and 50 °C hotter.
  • Cryo probe provides twice the sensitivity of a room temperature probe, allowing for high sensitivity measurements at lower analyte concentrations than a room temperature probe – e.g., 25% of the time for a typical 13C acquisition.
  • Diffusion probe allows for a 20-fold reduction in acquisition time – e.g., < 10 min. acquisition vs > 3 hours for a diffusion experiment on our current equipment-for low molecular weight species.
  • HRMAS package spins 5-6 times as fast as our current HRMAS probes, eliminating spinning side bands and include an auto-loading mechanism, eliminating the need to remove the probe to change samples

Varian DD2 600 MHz Spectrometer

This instrument is one of the specialty instruments. It is primarily used for biological samples and 1H, 13C, and multinuclear NMR spectroscopy. It is capable of 1D and 2D NMR experiments. It is capable of HRMAS and analysis of solid state samples, but is more difficult to book long term experiments than with the 500 MHz NMR spectrometer due to the demand for routine multinuclear analysis. HRMAS and solid state samples can be analyzed with prior notice (3 days). The ability to autotune to many nuclei makes this the instrument of choice for multinuclear NMR spectroscopy (Click here to see the available nuclei). This is the instrument of choice for variable temperature experiments.

Technical Information

  • Oxford 14.1T 51mm bore magnet
  • 4 channel console, 1 high band, 3 low band
  • Varian 40 channel shim set
  • Z-axis pulsed field gradients
  • 5 mm triple resonance probe, 1H detect {13C,15N} decouple–biological NMR, protein structural analysis
  • 5 mm broadband OneProbe, 15N to 31P tuning range (60 to 250 MHz), automatic tuning, and 12-position autosampler
  • 4mm Solid State NMR probe with high power High-band amplifier
  • Variable temperature: -80 °C to +90 °C

 

Bruker D8 Venture Single Crystal X-ray Diffractometer

This instrument is capable of measuring an x-ray diffraction pattern on a single crystal with either molybdenum or copper radiation. It is primarily used to aid in structural determination of a crystalline sample. It is capable of measuring data at room temperature and as low as 77K. Click here to receive access to the single crystal x-ray diffractometer.

 

 

 

 

Bruker D2 Phaser Powder X-ray Diffractometer

This instrument is capable of measuring an x-ray diffraction pattern on powder. It is primarily used to aid in structural determination of a powder samples. It is capable of measuring data at room temperature. Click here to receive access to the powder x-ray diffractometer.